| name | Hub | image | date Ceased operations | date Commenced operations | IATA Code | ICAO Code | city Headquarters | administrative division Headquarters | country Headquarters | continent Headquarters | Country | continent | Parent | number of aircrafts | number of destinations | Website |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ABA Air | 2005 | 1996 | ABP | Prague | Prague | Czech Republic | Europe | Czech Republic | Europe | Website | ||||||
| ABS Jets | Václav Havel Airport Prague |  | 2004 | ABP | Prague | Prague | Czech Republic | Europe | Czech Republic | Europe | 12 | Website | ||||
| Central Connect Airlines | Leoš Janáček Airport Ostrava |  | 2014 | 2005 | 3B | JBR | Ostrava | Moravian-Silesian Region | Czech Republic | Europe | Czech Republic | Europe | 2 | Website | ||
| Czech Airlines | Václav Havel Airport Prague |  | 06/10/1923 | OK | CSA | Prague | Prague | Czech Republic | Europe | Czech Republic | Europe | Korean air | 42 | 43 | Website | |
| Czech Connect Airlines | Brno–Tuřany Airport |  | 01/2012 | 2011 | CQ | CCG | Ostrava | Moravian-Silesian Region | Czech Republic | Europe | Czech Republic | Europe | 2 | 5 | Website |